ResolveDNA® WGA
Whole Genome Amplification Kits
- Minimum input amount needed as low as 4pg
- Uniform genome coverage and high fidelity
- High amplification efficiency with low allele drop-out rate
- Automatable workflow that enables higher sample processing at a lower cost per reaction
Challenges with Existing Whole Genome Amplification Methods
Legacy WGA techniques have suffered from amplification bias, poor uniformity, errors and artifacts, low genome coverage, inability to address all variant classes, low accuracy, poor reproducibility, and/or complex protocols that are difficult to automate or scale.
Novel Whole Genome Amplification
ResolveDNA WGA Kits utilize primary template-directed amplification (PTA) which is a novel, isothermal WGA method that reproducibly captures >95% of the genomes of single cells, in a controlled and more uniform and accurate manner than existing approaches. This improves variant calling sensitivity and specificity, lowers sequencing costs, and facilitates bioinformatic analysis.
Among all the whole-genome amplification (WGA) methods we have tested, PTA-based WGA has provided the most accurate output. It reliably stays close to the original 50/50 ratio of allele frequency in heterozygous loci, minimizing allele dropout and false-positive loss of heterozygosity."
Aleksei Mikhalchenko, PhD
Genome Informatics Lead, Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University